Review




Structured Review

SourceForge net imj macro
(A) RGB image showing DAPI staining of nuclei in blue, and phospho-histone3 staining in red. (B) shows the red channel processed with <t>IMJ</t> Edge to calculate the number of dividing cells. (C) shows the blue channel processed by IMJ Edge to identify all the nuclei. (D) shows the blue channel of (A) processed by IMJ Cai, OpenCFU and Cell Profiler. The detected nuclei are outlined in blue boxes by OpenCFU, and in red circles <t>by</t> <t>CellProfiler.</t> (E) shows the original Nomarski image of yeast cells, and the processed images by various methods next to it. (F) shows the Normarski image of a 293 cell and its processed versions next to it. The segmentation step was skipped in IMJ Edge to prevent segmentation of cell processes.
Imj Macro, supplied by SourceForge net, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/imj macro/product/SourceForge net
Average 90 stars, based on 1 article reviews
imj macro - by Bioz Stars, 2026-04
90/100 stars

Images

1) Product Images from "High-Throughput Method for Automated Colony and Cell Counting by Digital Image Analysis Based on Edge Detection"

Article Title: High-Throughput Method for Automated Colony and Cell Counting by Digital Image Analysis Based on Edge Detection

Journal: PLoS ONE

doi: 10.1371/journal.pone.0148469

(A) RGB image showing DAPI staining of nuclei in blue, and phospho-histone3 staining in red. (B) shows the red channel processed with IMJ Edge to calculate the number of dividing cells. (C) shows the blue channel processed by IMJ Edge to identify all the nuclei. (D) shows the blue channel of (A) processed by IMJ Cai, OpenCFU and Cell Profiler. The detected nuclei are outlined in blue boxes by OpenCFU, and in red circles by CellProfiler. (E) shows the original Nomarski image of yeast cells, and the processed images by various methods next to it. (F) shows the Normarski image of a 293 cell and its processed versions next to it. The segmentation step was skipped in IMJ Edge to prevent segmentation of cell processes.
Figure Legend Snippet: (A) RGB image showing DAPI staining of nuclei in blue, and phospho-histone3 staining in red. (B) shows the red channel processed with IMJ Edge to calculate the number of dividing cells. (C) shows the blue channel processed by IMJ Edge to identify all the nuclei. (D) shows the blue channel of (A) processed by IMJ Cai, OpenCFU and Cell Profiler. The detected nuclei are outlined in blue boxes by OpenCFU, and in red circles by CellProfiler. (E) shows the original Nomarski image of yeast cells, and the processed images by various methods next to it. (F) shows the Normarski image of a 293 cell and its processed versions next to it. The segmentation step was skipped in IMJ Edge to prevent segmentation of cell processes.

Techniques Used: Staining



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SourceForge net imj macro
(A) RGB image showing DAPI staining of nuclei in blue, and phospho-histone3 staining in red. (B) shows the red channel processed with <t>IMJ</t> Edge to calculate the number of dividing cells. (C) shows the blue channel processed by IMJ Edge to identify all the nuclei. (D) shows the blue channel of (A) processed by IMJ Cai, OpenCFU and Cell Profiler. The detected nuclei are outlined in blue boxes by OpenCFU, and in red circles <t>by</t> <t>CellProfiler.</t> (E) shows the original Nomarski image of yeast cells, and the processed images by various methods next to it. (F) shows the Normarski image of a 293 cell and its processed versions next to it. The segmentation step was skipped in IMJ Edge to prevent segmentation of cell processes.
Imj Macro, supplied by SourceForge net, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/imj macro/product/SourceForge net
Average 90 stars, based on 1 article reviews
imj macro - by Bioz Stars, 2026-04
90/100 stars
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(A) RGB image showing DAPI staining of nuclei in blue, and phospho-histone3 staining in red. (B) shows the red channel processed with IMJ Edge to calculate the number of dividing cells. (C) shows the blue channel processed by IMJ Edge to identify all the nuclei. (D) shows the blue channel of (A) processed by IMJ Cai, OpenCFU and Cell Profiler. The detected nuclei are outlined in blue boxes by OpenCFU, and in red circles by CellProfiler. (E) shows the original Nomarski image of yeast cells, and the processed images by various methods next to it. (F) shows the Normarski image of a 293 cell and its processed versions next to it. The segmentation step was skipped in IMJ Edge to prevent segmentation of cell processes.

Journal: PLoS ONE

Article Title: High-Throughput Method for Automated Colony and Cell Counting by Digital Image Analysis Based on Edge Detection

doi: 10.1371/journal.pone.0148469

Figure Lengend Snippet: (A) RGB image showing DAPI staining of nuclei in blue, and phospho-histone3 staining in red. (B) shows the red channel processed with IMJ Edge to calculate the number of dividing cells. (C) shows the blue channel processed by IMJ Edge to identify all the nuclei. (D) shows the blue channel of (A) processed by IMJ Cai, OpenCFU and Cell Profiler. The detected nuclei are outlined in blue boxes by OpenCFU, and in red circles by CellProfiler. (E) shows the original Nomarski image of yeast cells, and the processed images by various methods next to it. (F) shows the Normarski image of a 293 cell and its processed versions next to it. The segmentation step was skipped in IMJ Edge to prevent segmentation of cell processes.

Article Snippet: The IMJ macro, CellProfiler pipelines, and images are currently available from https://sourceforge.net/projects/cell-colony-edge/files/ .

Techniques: Staining